Experimental study of
effect on tonifying kidney herbs in pituitary ovary
adrenal gland of androgen sterilized rats
Acupuncture, Infertility, & IVF Research - Abstract Articles
Gui SQ; Yu J; Wei MJ Obstetrics and Gynecology
Hospital, Shanghai Medical University.
Chung Kuo Chung Hsi I Chieh
Ho Tsa Chih, 17(12):735-8 1997 Dec (ISSN: 1003-5370)
Objective: To observe
the effect of tonifying Kidney herbs (TKH) in pituitary,
ovary, adrenal gland of androgen sterilized rats (ASR).
Methods: ASR model
was established by injecting testosterone propionate
subcutaneously to SD female rats of 9 days age.
Morphological and hormonal change of pituitary, ovary
and adrenal gland in rats of 100-107 days old before and
after feeding TKH extract were observed by light and
electron microscope, cell culture immunohistochemical
studies and radioimmuno-assay.
Results: In the ASR groups, there were
intracytopiasmic lipid drops, autophagy, vacuole,
granulolysis of pituitary gland. There were anovulation
apparently and increased the amount of lipid drops in
cytoplasm of interstitial glandular cell of ovary. The
fatty drops of the reticular zone of adrenal gland
decreased, the number of AGNOR (P < 0.01) and PCNA (P <
0.01) increased significantly. The levels of FSH, LH (P
< 0.05-0.005) lowered, but the levels of DHA, T (P <
0.01, 0.05, 0.001) were raised significantly.
Morphological and hormonal change of all RSA returned to
normal range after the treatment.
Conclusions: The
hyperandrogenemia were disturbed by the function of sex
gland axis and adrenal in female rats of 9 days old and
played an important role in pathogenesis of ASR, the TKH
reduced the levels of androgen and induced ovulation
through sex gland axis and adrenal level and multi-organ
regulation.
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